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Efficacy and Downstream Compatibility of the Respiratory Sigma Collection Device
The pre-analytical phase of the total laboratory testing process is where the majority of laboratory errors occur and represents the most labour intensive, least standardized aspect of the process. Specimen processing is one of the most important steps in the pre-analytical phase as downstream reading, interpretation and reporting by technical staff is heavily dependent on the quality of specimen setup. In this study, the Copan SL™ solution device (Copan Italia; Brescia, Italy) and the MWE Sigma SP™ liquid collection
device containing a novel mucolytic agent were used.
The recovery of M. tuberculosis DNA at a constant level (CT 15-18) following storage of up to 48hrs at both room temperature and 4˚C was achieved for the MWE Sigma SP™ collection device. The Copan SL™ solution device showed variable results at both room temperature and at 4˚C.
The novel mucolytic agent demonstrated good mucolytic action and it enabled the emulsification of sputum and mucus resulting in a homogenous suspension allowing for easier, more consistent and reproducible planting and streaking of specimens. In addition, the Sigma SP™ collection device demonstrated compatibility with the FilmArray® system, as well as the BD MGIT™ system and Cepheid GeneXpert® for the detection of MTB complex.